Ultra violet (UV) spectroscopy, introduction,principle instrumentation,different types of uv transitionby mariomakhter@yahoo.com, • 1. Presentation topic – Ultra-violate spectroscopy Submitted by – Moriyom Akhter Md Shah Alam Department of pharmacy World university of Bangladesh • DEFINITION  UV RADIATION  PRINCIPLE OF UV-VIS SPECTROMETRY  THE ABSORPTION SPECTRUM  TYPES OF TRANSITIONS  ABSORBANCE LAWS  INSTRUMENTATION  EFFECT OF CHROMOPHORE  WOODWARD-FEISER RULE  SOLVENT EFFECTS  APPLICATIONS  • Ultraviolet and visible (UV-Vis) absorption spectroscopy is the measurement of the attenuation of a beam of light after it passes through a sample or after reflection from a sample surface.

Absorption measurements can be at a single wavelength or over an extended spectral range. Detection of functional groups. Detection of impurities Qualitative analysis Quantitative analysis Single compound without chromophore Drugs with chromophoric reagent It is helps to show the relationship between different groups, it is useful to detect the conjugation of the compounds • The region beyond red is called infra-red while that beyond violet is called as ultra –violet. The wavelength range of uv radiation starts at blue end of visible light(4000A) & ends at 2000A.

̊ •   Ultraviolet absorption spectra arise from transition of electron with in a molecule from a lower level to a higher level. A molecule absorb ultraviolet radiation of frequency (𝜗), the electron in that molecule undergo transition from lower to higher energy level.

UV -Vis spectroscopy Electronic absorption spectroscopy. Frank-Condon Principle • The nuclear motion (10-13 s) is negligible during the time required for an. Principles and applications of UV-visible spectroscopy Transmittance and absorbance When light passes through or is reflected from a sample, the amount of light absorbed is the difference between the incident radiation ( Io) and the transmitted radiation (I). The amount of light absorbed is expressed as either transmittance or absorbance.

Yaariyan 2014 Full Movie Torrent Download. Cara Membuat Denah Rumah Dengan Autocad 2010. PRINCIPLE AND APPLICATIONS. UV –VIS AND IR SPECTROSCOPY Ultraviolet–visible spectroscopy or ultraviolet-visible spectrophotometry.